Checklist
There are a lot of steps required to go from sample to species identification. Below you can find a 'checklist' to highlight all the steps I need to take to go from sample collection to species identification of parasitic nematodes. The steps will differ depending on your interests and focus, but seeing all the steps written out in this checklist can give you a starting point for your own experiments.
Collect faecal samples
Baermann technique
Microscopy
DNA extraction
Optional: DNA quality check
Optional: Gel electrophoresis
Nematode primers PCR
Gel electrophoresis
PCR cleanup
Computer and software check for sequencing
Flow cell check
MinION barcoding PCR
PCR cleanup
Optional: gel electrophoresis
DNA quality check
MinION library preparation
MinION run
Wash flow cell
Bioinformatics